Bukki is the son of Abishua, the grandson of Pinechas, and is the fifth high priest of the people of Israel. His ancestors are Aaron, the priest and Levi. Family tree because it provides most basic information of all: the sequence of nucleotides. This knowledge allows, for example, to detect regulatory and gene sequences, to identify comparisons between homologous genes between species and mutations.
Sanger's method is based on the use of di-deoxynucleotides (ddNTPs) in addition to the normal nucleotides (NTPs) found in DNA. These modified nucleotides, when integrated into a sequence, prevent the addition of further nucleotides. For the DNA to be sequenced, it must be denatured in some wires using heat. Subsequently, a primer is connected to one of the template wires. This primer is constructed such that its 3 'end lies next to the DNA sequence that is of interest. The primer or one of the nucleotides must be radioactive or fluorescently labeled so that the end product can be detected on a gel.
A similar method is to color the ddNTPs with different dyes. These dyes fluoresce at different wavelengths read by a machine. The result is a peek pattern that shows the sequence of the DNA.
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