The Ussing Chamber is a device used to measure the transport of ions through the epithelial tissue.
The room is named after the Danish zoologist Hans Ussing (1911-2000), who invented it in the 1950's.
A Ussing chamber consists of two compartments separated from the epithelium (epithelial epithelium or monolayer epithelial cells grown on permeable supports) under examination. Epithelials are composed of polarized cells, that is, they have an apical or mucosal side and a basolateral or serous side. In a Ussing room you can isolate the apical side from the basolateral side. The two rooms are filled with equal amounts of Ringer's solution. In each epithelium there is a transport of ions that occurs from the apical to the basolateral side and vice versa. This ion transport produces a potential difference through epithelium. The generated power difference is measured using two electrodes located near the epithelial tissue. Bibliografiamodifica wikitesto
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