Column in an HPLC setup
A column can be used in chemistry by chromatography to separate the components into a mixture. The one column uses the polarity of the components, another of the magnitude of the molecules.
Stationary phaseThe stationary phase of a column is the inner lining of the tube. This coating is responsible for the separation of the components. Mobile phase
The mobile phase is a liquid or gas that moves through the tube along the stationary phase. A column can occur in different types to be used for different analyzes. Gepakte column
A packed column is a thick short column where a stationary phase is in granular form. The grains of the stationary phase are sometimes irregular in shape, but in most cases the grains have a fixed shape and size. Capillaire column
A capillary column is a long thin column (up to 150 meters). The stationary phase is applied as a coating on the wall of the column. Dish number
The dish number is a measure of the separation power of a column. The larger the dish number of a column, the better the separation of the column in question will be. The dish number is column dependent, a long column will have more dishes than a short column. The dish number is also quality-dependent. If 2 same columns have different dish numbers, then the column with the largest number of dishes is the better quality column. So the dish number is also a good indication to check the quality of columns. Dish Height
The dish height is derived from the dish number. Dish Height = (Length Column / Dish Number). The dish height is a better indication of the quality of the column than the dish number. This because a long column has a larger dish number than a short column. With the tray height, the quality of columns of different lengths can be compared to each other.
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